All posts by alischachtschneider

Biofabricate Symposium

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

BIOFABRICATE_ExhibitorDescriptionForm_Page_1

http://www.biofabricate.co

Check out the website!!

Annelie and I were both involved in Biofabricate last week. The event was a full day of talks and workshops with speakers involved in biofabrication of future materials for products and industry.

Annelie helped to organize the event and I worked with Genspace in designing and setting up  a pop up lab for people to come and interact with some of the material being talked about i the conference.

Artists and designers had projects displayed which used grown material and the building blocks life to  manufacture objects and material.

I had some work displayed within the pop up labs exhibition area of Genspace members projects.

 

IMG_8680

IMG_8690

IMG_8662

Genspace Pop Up Lab

We wanted to create a space where people could come and interact with some of the materials being presented in the conference.

 

 

 

 

IMG_8753

Fungal Shoes

How can we utilize fashion to create new understandings of the bodies interaction with biomaterials?

Fungal Shoes offer a new foot fungus; a type of foot covering that can begin to transform our relationship with fungi. Negative connotations of feet and fungus can be reshaped through the growth of mycelium shoes, allowing for new sustainable methods to create objects accepted within culture. Fungal shoes were grown in the lab, using a mycelium culture grown using DIY methods from a grocery store mushroom. They are displayed in progress to highlight the process of growth and show the steps involved in mycoproduction.

Beyond: Mycelium

Ali and Annelie- Final Project:

Beyond: Mycelium
What we’ve explored:

We  have created a DIY magnetic stirrer to oxygenate our cultures

using a laser cut plexiglass box, a computer fan some magnetics and a bit of tinkering, we’ve designed an efficient, cheap stirrer to provide oxygen to our growing cultures.

We’ve explored and designed different growth substrates using different sources of sugars  and nutrients

Explored the interaction between fungi and electricity (see gallery) based on research on the relationship in nature between fungi and lightning, this research has driven us to continue exploring this. We created different set ups to create a current through the agar medium in petri dishes we inoculated with mycelia.

The preliminary results of this research indicate that a plate modified with electric current from a 9V battery has increased the growth rate compared to non electrified cultures and a culture with similar set up that has not had the electric current for as long a time. We’ve also created a plate modified with magnetic filings and used a magnet to affect the culture.
_HI_9059-1 copy

We will continue to go forward with this research and will possibly look to an electrical engineer to hep analyze and create an ideal set up for this experiment. We may look for the scar caused by lightning to develop similarly in the mycelia to the scars that form in nature and on skin, but need to further research this phenomenon.

We’ve explored briefly the use of molecular gastronomy to create knew methods of growth. We used alginate and calcium lactate to attempt spherification of the media which will be inoculated with mycelium.

We will continue to grow our liquid cultures and hope to in the future grow more material to begin to understand how we will use it.

Research document:

myceliumresearchdocument

 

What is Mycelium?

“Mycelium is the vegetative part of fungi, which consists of a network of interconnected filamentous cells called hyphae. The mycelium of mushroom- forming basidiomycetes is highly attractive and embodies a great potential, because of its tendency of growing on a wide variety of substrates, therefore resulting into a range of diverse materials and applications, related to the architecture and the design fields. Moreover, this organic network of filamentous cells is characterised by peculiar properties, such as strength, elasticity, thickness, homogeneity and water repellency.”

What is currently be done with mycelium?

Currently mycelium is being used in the art and design world for various applications. The strong fibers of mycelium works wells as a natural alternative to wood, cork and plastics and can also be easily shaped into both structural materials such as insulation and decorative artifacts such as lampshades and homeware. It is also produced in a more energy efficient way than conventional manufacturing.

What we would like to explore?

The use of mycelium as an alternative building material is revolutionary and is proving that there are natural alternatives to our current ways of manufacturing, but within the discipline of mycoculture itself there has not been much experimentation and it seems that the majority are using the fibres in a composite of materials and the actual chemistry beyond the physiology is not being explored.

We would like to see how we can go beyond the current methods of growing and using mycelium cultures and with this explore new material solutions. We hope to achieve this through a series of experiments addressing these two parts of mycoculture:

  1. Growing Mycelium
  2. Fabricating with Mycelium

Growing Mycelium

Current research with mycelium involves the growth of material in organic decaying substrates. We propose an alternative approach, we will be growing pure mycelium in a liquid culture using experimentally designed methods, based on research in the industrial production of mycelium for medicinal use.

After our tour to the Industry City Distillery we have been doing a lot of research into growth optimisation and found that the same alginate that is used to keep the yeast growing at optimal temperature and Ph level can be used as a substrate for the growth of mycelium in liquid culture. We will definitely be exploring this avenue when we get to the growing of larger masses of mycelium.

We will also be prototyping a DIY bioreactor to further optimize the growth of the material.

Fabricating with Mycelium

Fungi and electricity:

Lightning induces fruiting of mushrooms in nature

We would like to scale down this interaction between fungi and electricity by creating a modified petri dish experiment that will test the effect of electrical current on the growth of fungal cultures.

There is also currently some interest within the science world in the perceived conductivity of mycelium. As per our previous project we would like to continue this research with more scientific backing.

Fungi as fabric:

We are hoping to move away from the composite use of mycelium to explore the chemical makeup of the hyphae and see if there is a way in which we can use this fibre for fabric or as alternative to cotton or yarn.

 

The cultures:

Reishi

myclium-04 IMG_8930 IMG_8933

 

 

Ghost fungi

myclium-03 myclium-06

IMG_8935

 

 

Chicken of the Woods

IMG_8349 IMG_0751

IMG_8938

 

 

Shiitake

IMG_8927

 

Mycelium + Electricity

 

Modifying the Jar:
Creating the ideal vessel for liquid cultures

 

Oxygenating cultures: the magnetic stirrer

IMG_8338 copy

 

[We will be creating a diy magnetic stirrer to facilitate the growth of mycelium liquid cultures within an incubator box to achieve the ideal temperature for the organisms. This drawing is a box that has a computer fan inside with magnets attached, then a magnetic bar is placed within the liquid culture and when placed on the box, the stirring is produced from the rotations of the fan. This design allows the stirrer to be portable, and we can create a setup with multiple fans set up to allow us to stir many cultures at one time in a controlled setting.

Making the special containers for growth of mycelium. The jars are modified with two holes in the lid, one is stuffed with poly fill filling and the other is filled with RTV (autoclavable) silicone. The silicone is a seth healing injection port for the insertion of syringe needles, and the polyfill acts as a filter allowing oxygen into the jar.

And lastly, we have begun to create a modified petri dish setup with which we will test fungi’s response to electricity. We are interested in this after reading about fungi’s relationship with lightning and we are looking to simulate this interaction in the lab.]

IMG_8789 IMG_8792IMG_8897 IMG_8896 dried samples

 

 

 

Beyond: Final Project Update

This week Annelie and I have been designing our experiments, our nutrients and our vessels and tools for the growth of mycelium in liquid medias.

We will be creating a diy magnetic stirrer to facilitate the growth of mycelium liquid cultures within an incubator box to achieve the ideal temperature for the organisms. This drawing is a box that has a computer fan inside with magnets attached, then a magnetic bar is placed within the liquid culture and when placed on the box, the stirring is produced from the rotations of the fan. This design allows the stirrer to be portable, and we can create a setup with multiple fans set up to allow us to stir many cultures at one time in a controlled setting.

IMG_8406 copy IMG_8405 copy

Making the special containers for growth of mycelium. The jars are modified with two holes in the lid, one is stuffed with poly fill filling and the other is filled with RTV (autoclavable) silicone. The silicone is a seth healing injection port for the insertion of syringe needles, and the polyfill acts as a filter allowing oxygen into the jar.

IMG_8412 copyThis is a sample of dried mycelium that has been sterilized and becomes a kind of ‘fabric’.

Our cultures on the stirrer: batch 2

https://drive.google.com/a/newschool.edu/?tab=mo#my-drive

Documentation and lab notes:

Mycelium Report

 

And lastly, we have begun to create a modified petri dish setup with which we will test fungi’s response to electricity. We are interested in this after reading about fungi’s relationship with lightning and we are looking to simulate this interaction in the lab.

Beyond: Final Project Proposal

What is Mycelium?

“Mycelium is the vegetative part of fungi, which consists of a network of interconnected filamentous cells called hyphae. The mycelium of mushroom- forming basidiomycetes is highly attractive and embodies a great potential, because of its tendency of growing on a wide variety of substrates, therefore resulting into a range of diverse materials and applications, related to the architecture and the design fields. Moreover, this organic network of filamentous cells is characterised by peculiar properties, such as strength, elasticity, thickness, homogeneity and water repellency.”

What is currently be done with mycelium?

Currently mycelium is being used in the art and design world for various applications. The strong fibers of mycelium works wells as a natural alternative to wood, cork and plastics and can also be easily shaped into both structural materials such as insulation and decorative artifacts such as lampshades and homeware. It is also produced in a more energy efficient way than conventional manufacturing.

What we would like to explore?

The use of mycelium as an alternative building material is revolutionary and is proving that there are natural alternatives to our current ways of manufacturing, but within the discipline of mycoculture itself there has not been much experimentation and it seems that the majority are using the fibres in a composite of materials and the actual chemistry beyond the physiology is not being explored.

We would like to see how we can go beyond the current methods of growing and using mycelium cultures and with this explore new material solutions. We hope to achieve this through a series of experiments addressing these two parts of mycoculture:

  1. Growing Mycelium
  2. Fabricating with Mycelium

Growing Mycelium

Current research with mycelium involves the growth of material in organic decaying substrates. We propose an alternative approach, we will be growing pure mycelium in a liquid culture using experimentally designed methods, based on research in the industrial production of mycelium for medicinal use.

After our tour to the Industry City Distillery we have been doing a lot of research into growth optimisation and found that the same alginate that is used to keep the yeast growing at optimal temperature and Ph level can be used as a substrate for the growth of mycelium in liquid culture. We will definitely be exploring this avenue when we get to the growing of larger masses of mycelium.

We will also be prototyping a DIY bioreactor to further optimize the growth of the material.

Fabricating with Mycelium

Fungi and electricity:

Lightning induces fruiting of mushrooms in nature

We would like to scale down this interaction between fungi and electricity by creating a modified petri dish experiment that will test the effect of electrical current on the growth of fungal cultures.

There is also currently some interest within the science world in the perceived conductivity of mycelium. As per our previous project we would like to continue this research with more scientific backing.

Fungi as fabric:

We are hoping to move away from the composite use of mycelium to explore the chemical makeup of the hyphae and see if there is a way in which we can use this fibre for fabric or as alternative to cotton or yarn.

21.The-Future-of-Plastic-©Officina-Corpuscoli-Maurizio-Montalti-Pure-Mycelium-Experiments-Growing-Lab-Overview

Maurizio Montalti

http://www.corpuscoli.com/projects/the-future-of-plastic/

 

Jonas-Edvard-Myx-Mushroom-Lamp-5-537x405

Jonas Edvard Nielsen

http://www.designboom.com/design/jonas-edvard-myx-lamps-mushroom-mycelium-09-02-2014/

 

roundmushroom-600x433

Phil Ross

http://glasstire.com/2012/09/08/the-future-is-fungal-interview-with-phil-ross/ 

 

shake1

http://openwetware.org/wiki/DIYbio/FAQ/Projects#Fermentors.2C_Bioreactors.2C_Photo_Bioreactors 

 

https://docs.google.com/a/newschool.edu/file/d/0B9eBTLIkfDZYZVZ3N2NQWDRpQnM/edit

https://docs.google.com/a/newschool.edu/file/d/0B9eBTLIkfDZYazlSMlF6aktFVTg/edit

https://docs.google.com/a/newschool.edu/file/d/0B9eBTLIkfDZYODdUbUFlUmlYcWc/edit

 

DIY Microscope

Here is the first microscope design I created. This initial model is just a version that will be refined for a final design. I like the acrylic and metal hardware but want to refine the overall shape as well as the system for focusing the camera

IMG_7874

IMG_7876

And here are some images taken with the microscope…

1-3. Images of hydra

Photo on 10-30-14 at 5.16 PM

Photo on 10-9-14 at 8.00 PM #2

Photo on 10-30-14 at 5.14 PM #2

 

Photo on 10-30-14 at 2.47 PM

4. surface of my tongue with interesting effect from camera

Photo on 10-30-14 at 5.25 PM

5. surface of dried mycelium

 

DIY decellularization mobile lab

photo 3-4

 

Mobile decellularization lab

My goal with this lab is to create a mobile lab to repurpose wasted and unused plant and animal tissues to create pieces of decellularized tissue scaffolds. These can then be experimented with for artistic purposes. I am interested in the interaction between the body, technology and biology.  Through decellularizing tissues,  I’d like to understand what the body does with those tissues and how they interact. How could we wear these scaffolds in the future, essentially creating something from trash?  What would a device look like that we wear to decellularize tissues as we move?

This is a quick prototype of the decellularization setup. The containers that will hold the tissue being decellularized has a pump attached to allow fresh detergent solution to be pumped in.

The lab will include multiple glass or polypropylene containers to hold the detergent solution, water, and other liquids to remove the cellular contents from the tissues, tools to handle the materials, and various devices to apply the detergents with and to affect the process- possibly a diy agitator.

The process involves washing the tissue, and soaking it in a lab detergent solution (sodium dodecyl sulfate-SDS). Over time the detergent removes the cellular contents from the tissue leaving only the extra cellular matrix (ECM) remaining. The ECM is a scaffold of either collagen (from animal tissue) or cellulose (from plant tissue). This scaffold can then be used as a scaffold to grow living cells. (i won’t be reseeding the tissues)

Some inspiration:

The Pelling Lab has done extensive work with tissue decellularization- http://www.pellinglab.net/?s=decell

A scientific paper with a description of the protocol-http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084613/#!po=29.6875

tumblr_ls14x5whHu1qed0my

a bioreactor setup at Pelling lab

tumblr_ls8ylxPotH1qed0my

a piece of decellularized steak

Hi, I’m Ali

IMG_6369

Hello I’m Ali,

I am a 4th year BFA Fashion Design student focusing on bioart, fashioning bodies, and the relationship between the body and technology. I view fashion in the larger context of making or fashioning objects and as social practice. As a former ballet dancer, I am interested in the relationship of the body, other living systems and environments. I am currently a member of Genspace, the community biotechnology lab, where I am working on my thesis and growing biomaterials. I am very interested in DIYbio, hacking and subverting science, interaction and growing organisms and biomaterials to begin to explore the implications of science and technology in society,  and to increase interest in science in everyday life.  At Genspace, I am currently growing the symbiotic colony of bacteria and yeast in a kombucha tea media, and mycelium- the vegetative part of fungi. I am growing these materials to begin to understand how the they interact with the body, and what they can do, as well as how they can be investigated, experimented with, and grown.

Some Inspiration:

 

SemiDollG

Semi Living Worry Dolls, Tissue Culture and Art Project (Oron Catts and Ionat Zurr)

Based on the old Guatemalan story told to children- that any worries should be whispered to your dolls. At night, you can tell your dolls your worries and they will solve them while you sleep. Oron and Ionat created worry dolls for the public to whisper their worries(possibly of biotechnology and the dolls themselves) to. These were the first engineered tissue sculptures presented in an art gallery. The dolls were hand made from a degradable polymer and then seeded with cells from the McCoy cell line. During the exhibition they were displayed in bioreactors to sustain growth, the dolls were nourished daily, and at the end they were ‘killed’.  http://tcaproject.org/semi-living-worry-dolls/

 

BqquekZCUAAvhcW

Invisible Resources, Zuzana Gombosova

Zuzana used bacteria which produce bacterial cellulose, she experimented with growth by creating a device which experimented with different patterns of nourishing and feeding. Her goals were to raise the questions of how devices can alter perception of products and how the patience required to grow material might change our material culture. http://www.zuzana-gombosova.squarespace.com/#/new-gallery/